Inhibition of Mayaro virus replication by prostaglandin A1 and B2 in Vero cells

The effect of prostaglandins (PGA1 and PGB2) on the replication of Mayaro virus was studied in Vero cells. PGA1 and PGB2 antiviral activity was found to be dose-dependent. However, while 10 µg/ml PGB2 inhibited virus yield by 60 percent, at the same dose PGA1 suppressed virus replication by more than 90 percent. SDS-PAGE analysis of [35S]-methionine-labelled proteins showed that PGA1 did not alter cellular protein synthesis. In infected cells, PGA1 slightly inhibited the synthesis of protein C, while drastically inhibiting the synthesis of glycoproteins E1 and E2

Effect of hemodialysis on protein C and antithrombin III

The effect of hemodialysis on natural coagulation inhibitors including protein C [PC] and antithrombin III [AT III] had been studied. Plasma PC and AT III levels measured in 20 uremic patients on maintenance hemodialysis immediately before and after dialysis treatment. These values were compared with those obtained from 20 matched healthy controls. Functional activities of PC was determined by coagulometric method and functional activities of AT III was determined by amidolytic method. Both the functional activities of PC and AT III in uremic patients were significantly lower than those of controls. No significant change in the level of AT III was observed with dialysis, but a progressive increase of functional activity of PC was documented with hemodialysis. On the luisis of this study, the risk of thrombosis may increase due the decrease in AT III levels. However, this effect may be compensated by the increase in PC activity, possibly due to the removal of an inhibitory substance on PC activity